The Infowars host and Republican Senator battled it out on Capitol Hill Wednesday morning.
Rubio blamed Russian President Vladimir Putin for enabling the attacks.
The anti-LGBT Florida senator lives to see another term.
The Florida senator opposes LGBT rights and yet lectures Christians for judging LGBT Americans.
The LGBT community is fuming that the American Renewal Project is hosting its gathering — with Trump and Rubio in attendance — in the same city where 49 people were murdered at a gay nightclub weeks earlier.
"Leave it to the media and liberal activists to label a gathering of faith leaders as an anti-LGBT event," said the Florida senator.
The senator was called "Florida's most brazen political opportunist" by a local alt-weekly.